Insilico Analysis of Phytoconstituents from Allium sativum as Potential Inhibitors of Inha in Mycobacterium tuberculosis

ABSTRACT Tuberculosis is leading cause of death among the global bacterial infections. The main causative for tuberculosis is Mycobacterium tuberculosis, which will survive in its host human being for decades in latent or chronic levels. In addition, the late multidrug resistance at a disturbing rate accompanies the appearance of tuberculosis. The quick spread of resistance to initial stage treatment medications has redirected the focus of the medical community in the creation of an array of new drug against Mycobacterium tuberculosis. The InhA protein is a component of Fatty acid synthetase (FAS) II and exhibits an NADH reliant enoyl-ACP reductase activity. InhA is a vital enzyme of M.tuberculosis in control of cell wall synthesis, which can turn out to be a great focus for the synthesis of anti-tubercular treatment. Inspired from the offering biological actions of phytoconstituents from Allium sativum, the current research concentrates on looking at novel lead compounds from the plant. Molecular docking studies were carried out employing specific phytoconstituents from A.sativum with the protein InhA target. Ajoene shows much more encouragingresults with a Mol Dock rating of 80.6047Kcal/mol, as opposed to the typical initial line drug isoniazid (Moldock score: -58.7028 Kcal/mol). Molecular docking prediction indicate that Ajoene could be formulated into a possible treatment drug for Mycobacterium tuberculosis.

A sustained release of tablet granules associated with ZnS nanocrystals using Tamarind seed polysaccharide.

Tamarind seed polysaccharide (TSP) isolated from tamarind kernel powder was investigated for sustained release manners of salicylic acid drug. Tablet granules of salicylic acid were prepared, with two different grades of TSP and Cross linked TSP and embedded with chemically synthesized ZnS nanocrystals. Five different formulations made and the drug excipient mixtures were subjected to pre-formulation studies such as physicochemical studies, in vitro dissolution test, disintegration test, angle of repose and drug content. The physicochemical properties of tablets were found within the limits. Formulation F1 and F5 containing TSP and Cross linked were found to release the drug in sustained manner up to 24 hour and were stable under accelerated conditions of temperature for 6 months since there were no significant changes in drug content and physical parameters. This formulation was more comfortable to the user due to less erosion, faster and optimum pH of surrounding medium.